assume Q1:
A _\_ B ={}
; :: thesis: contradiction consider A1, A2 being Subset of U such that p1:
( A1 c= A2 & A =Inter (A1,A2) )
byU2; consider B1, B2 being Subset of U such that p2:
( B1 c= B2 & B =Inter (B1,B2) )
byU2; consider y being set such that q4:
y = A1 \ B1
;
( not A1 in A or not B1 in B or not y = A1 \ B1 )
bySETFAM_1:def 6, Q1; hence
contradiction
byq4, p1, p2; :: thesis: verum